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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
20/02/2013 |
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Data da última atualização: |
10/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARÃES, D. A.; GARCIA, S. C. G. de; FERREIRA, M. A. P.; SILVA, S. do S. B. da; ALBUQUERQUE, N. I. de; LE PENDU, Y. |
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Afiliação: |
DIVA ANELIA GUIMARÃES, UFPA; SYLVIA CRISTINA GARCIA DE GARCIA, UFPA; MARIA AUXILIADORA PANTOJA FERREIRA, UFPA; SULEIMA DO SOCORRO BASTOS DA SILVA, UFPA; NATALIA INAGAKI DE ALBUQUERQUE, CPATU; YVONNICK LE PENDU, UNIVERSIDADE DE COSTA RICA. |
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Título: |
Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Revista de Biologia Tropical, v. 60, n. 1, p. 437-445, Mar. 2012. |
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ISSN: |
0034-7744 |
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Idioma: |
Inglês |
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Conteúdo: |
The sustainability and production of collared peccary (Pecari tajacu) has been studied in the last few years; however, further information on its reproduction is necessary for breeding systems success. Understanding folliculogenesis aspects will contribute to effective reproductive biotechniques, which are useful in the preservation and production of wildlife. The aim of this study was to evaluate the ovarian folliculogenesis in collared peccary. Ovaries from six adult females of collared peccary were obtained through ovariectomy and analyzed. These were fixed in aqueous Bouin?s solution and sectioned into 7µm slices, stained with hematoxilin-eosin and analyzed by light microscopy. The number of pre-antral and antral follicles per ovary was estimated using the Fractionator Method. The follicles, oocytes and oocyte nuclei were measured using an ocular micrometer. Results showed that the length, width, thickness, weight, and the gross anatomy of the right and left ovaries were not significantly different. However, the mean number of corpora lutea was different between the phases of the estrous cycle (p<0.05), with the highest mean in the luteal phase. Primordial follicles were found in the cortex; the oocytes were enveloped by a single layer of flattened follicular cells. In the primary follicles, proliferation of the follicular cells gave rise to cuboidal cells (granulosa cells). The secondary follicle was characterized by two or more concentric layers of cuboidal cells (granulosa), beginning of antrum formation, and the presence of pellucid zone and theca cells. Antral follicles were characterized by a central cavity (antrum), the presence of cumulus oophorus and theca layers (interna and externa). In the right ovary, the values of the primordial and primary follicles were similar, but significantly different from the secondary ones (p<0.05). In the left ovary, significant differences were observed between all follicles in the follicular phase (p<0.05); the mean number of primordial and primary follicles was similar in the luteal phase. The mean number of pre-antral follicles and antral follicles in the follicular phase was higher in the left ovary (p<0.05). The mean number of antral follicles in the luteal phase was similar in both ovaries. We also found significant differences in mean diameter of preantral follicles, oocyte, granulosa layer and oocyte nucleus during the estrous cycle. In the antral follicles a significant difference was observed only in follicular diameter (p<0.05). The predominance of active primordial and primary follicles was found in both phases; otherwise the secondary follicles and antral follicles showed a high degree of degeneration. The results obtained in the present work will strengthen the development of biotechnology programs to improve the productive potential and conservation of the collared peccary MenosThe sustainability and production of collared peccary (Pecari tajacu) has been studied in the last few years; however, further information on its reproduction is necessary for breeding systems success. Understanding folliculogenesis aspects will contribute to effective reproductive biotechniques, which are useful in the preservation and production of wildlife. The aim of this study was to evaluate the ovarian folliculogenesis in collared peccary. Ovaries from six adult females of collared peccary were obtained through ovariectomy and analyzed. These were fixed in aqueous Bouin?s solution and sectioned into 7µm slices, stained with hematoxilin-eosin and analyzed by light microscopy. The number of pre-antral and antral follicles per ovary was estimated using the Fractionator Method. The follicles, oocytes and oocyte nuclei were measured using an ocular micrometer. Results showed that the length, width, thickness, weight, and the gross anatomy of the right and left ovaries were not significantly different. However, the mean number of corpora lutea was different between the phases of the estrous cycle (p<0.05), with the highest mean in the luteal phase. Primordial follicles were found in the cortex; the oocytes were enveloped by a single layer of flattened follicular cells. In the primary follicles, proliferation of the follicular cells gave rise to cuboidal cells (granulosa cells). The secondary follicle was characterized by two or more concentric layers of cuboidal cells (gran... Mostrar Tudo |
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Palavras-Chave: |
Pecari tajacu. |
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Thesagro: |
Anatomia animal; Morfologia animal; Ovario. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/76842/1/32-Guimaraes-Collared-peccary.pdf
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Marc: |
LEADER 03620naa a2200241 a 4500
001 1950273
005 2022-11-10
008 2012 bl uuuu u00u1 u #d
022 $a0034-7744
100 1 $aGUIMARÃES, D. A.
245 $aOvarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla$bTayassuidae).$h[electronic resource]
260 $c2012
520 $aThe sustainability and production of collared peccary (Pecari tajacu) has been studied in the last few years; however, further information on its reproduction is necessary for breeding systems success. Understanding folliculogenesis aspects will contribute to effective reproductive biotechniques, which are useful in the preservation and production of wildlife. The aim of this study was to evaluate the ovarian folliculogenesis in collared peccary. Ovaries from six adult females of collared peccary were obtained through ovariectomy and analyzed. These were fixed in aqueous Bouin?s solution and sectioned into 7µm slices, stained with hematoxilin-eosin and analyzed by light microscopy. The number of pre-antral and antral follicles per ovary was estimated using the Fractionator Method. The follicles, oocytes and oocyte nuclei were measured using an ocular micrometer. Results showed that the length, width, thickness, weight, and the gross anatomy of the right and left ovaries were not significantly different. However, the mean number of corpora lutea was different between the phases of the estrous cycle (p<0.05), with the highest mean in the luteal phase. Primordial follicles were found in the cortex; the oocytes were enveloped by a single layer of flattened follicular cells. In the primary follicles, proliferation of the follicular cells gave rise to cuboidal cells (granulosa cells). The secondary follicle was characterized by two or more concentric layers of cuboidal cells (granulosa), beginning of antrum formation, and the presence of pellucid zone and theca cells. Antral follicles were characterized by a central cavity (antrum), the presence of cumulus oophorus and theca layers (interna and externa). In the right ovary, the values of the primordial and primary follicles were similar, but significantly different from the secondary ones (p<0.05). In the left ovary, significant differences were observed between all follicles in the follicular phase (p<0.05); the mean number of primordial and primary follicles was similar in the luteal phase. The mean number of pre-antral follicles and antral follicles in the follicular phase was higher in the left ovary (p<0.05). The mean number of antral follicles in the luteal phase was similar in both ovaries. We also found significant differences in mean diameter of preantral follicles, oocyte, granulosa layer and oocyte nucleus during the estrous cycle. In the antral follicles a significant difference was observed only in follicular diameter (p<0.05). The predominance of active primordial and primary follicles was found in both phases; otherwise the secondary follicles and antral follicles showed a high degree of degeneration. The results obtained in the present work will strengthen the development of biotechnology programs to improve the productive potential and conservation of the collared peccary
650 $aAnatomia animal
650 $aMorfologia animal
650 $aOvario
653 $aPecari tajacu
700 1 $aGARCIA, S. C. G. de
700 1 $aFERREIRA, M. A. P.
700 1 $aSILVA, S. do S. B. da
700 1 $aALBUQUERQUE, N. I. de
700 1 $aLE PENDU, Y.
773 $tRevista de Biologia Tropical$gv. 60, n. 1, p. 437-445, Mar. 2012.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/10/2010 |
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Data da última atualização: |
25/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
KULCHESKI, F. R.; MARCELINO-GUIMARAES, F. C.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; MARGIS, R. |
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Afiliação: |
FRANCELI RODRIGUES KULCHESKI, UFRGS; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; ROGÉRIO MARGIS, UFRGS. |
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Título: |
The use of microRNAs as reference genes for quantitative polymerase chain reaction in soybean. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Analytical Biochemistry, Amsterdam, v. 406, n. 2, p. 185-192, nov. 2010. |
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DOI: |
10.1016/j.ab.2010.07.020 |
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Idioma: |
Português |
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Conteúdo: |
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, the choice of a suitable gene to use as an internal control is a crucial factor. These genes, such as housekeeping genes, should have a constant expression level in different tissues and across different conditions. The advances in genome sequencing have provided high-throughput gene expression analysis and have contributed to the identification of new genes, including microRNAs (miRNAs). The miRNAs are fundamental regulatory genes of eukaryotic genomes, acting on several biological functions. In this study, miRNA expression stability was investigated in different soybean tissues and genotypes as well as after abiotic or biotic stress treatments. The present study represents the first investigation into the suitability of miRNAs as housekeeping genes in plants. The transcript stability of 10 miRNAs was compared to those of six previously reported housekeeping genes for the soybean. In this study, we provide evidence that the expression stabilities of miR156b and miR1520d were the highest across the soybean experiments. Furthermore, these miRNAs genes were more stable than the most commonly protein-coding genes used in soybean gene expression studies involving RT-qPCR. |
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Palavras-Chave: |
Reação em cadeia da polimerase. |
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Thesagro: |
Gene; RNA; Soja. |
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Thesaurus NAL: |
Genes; MicroRNA; Quantitative polymerase chain reaction; Soybeans. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02220naa a2200277 a 4500
001 1864539
005 2017-07-25
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1016/j.ab.2010.07.020$2DOI
100 1 $aKULCHESKI, F. R.
245 $aThe use of microRNAs as reference genes for quantitative polymerase chain reaction in soybean.
260 $c2010
520 $aReverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, the choice of a suitable gene to use as an internal control is a crucial factor. These genes, such as housekeeping genes, should have a constant expression level in different tissues and across different conditions. The advances in genome sequencing have provided high-throughput gene expression analysis and have contributed to the identification of new genes, including microRNAs (miRNAs). The miRNAs are fundamental regulatory genes of eukaryotic genomes, acting on several biological functions. In this study, miRNA expression stability was investigated in different soybean tissues and genotypes as well as after abiotic or biotic stress treatments. The present study represents the first investigation into the suitability of miRNAs as housekeeping genes in plants. The transcript stability of 10 miRNAs was compared to those of six previously reported housekeeping genes for the soybean. In this study, we provide evidence that the expression stabilities of miR156b and miR1520d were the highest across the soybean experiments. Furthermore, these miRNAs genes were more stable than the most commonly protein-coding genes used in soybean gene expression studies involving RT-qPCR.
650 $aGenes
650 $aMicroRNA
650 $aQuantitative polymerase chain reaction
650 $aSoybeans
650 $aGene
650 $aRNA
650 $aSoja
653 $aReação em cadeia da polimerase
700 1 $aMARCELINO-GUIMARAES, F. C.
700 1 $aNEPOMUCENO, A. L.
700 1 $aABDELNOOR, R. V.
700 1 $aMARGIS, R.
773 $tAnalytical Biochemistry, Amsterdam$gv. 406, n. 2, p. 185-192, nov. 2010.
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